Synergistic Effects of Arsenic Trioxide and Radiation: Triggering the Intrinsic Pathway of Apoptosis

Authors

  • Ali Neshasteriz Radiation biology research center, Iran University of medical sciences, Tehran, Iran
  • Arshad Hosseini Department of Medical Biotechnology, Faculty of allied Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Asaad Azarnezhad Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
  • Elahe Mirzaei Microbiology Department, Faculty of Science, Islamic Azad University, Tehran, Iran
  • Kave Moloudi Radiation Sciences Department, Faculty of allied Medicine school, Iran University of Medical Sciences, Tehran, Iran
  • Mehdi Shomali Radiology Department, Faculty of allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Nazila Eyvazzadeh Radiation Research Center, Faculty of Paramedicine, AJA University of Medical sciences, Tehran, Iran
  • Samira Eynali Medical physics and Biomedical Engineering Department, school of Medicine, Tran University of Medical Sciences, Tehran, Iran
Abstract:

Background: Arsenic trioxide (ATO) has been reported as an effective anti-cancer and a US Food and Drug Administration (FDA) approved drug for treatment of some cancers. The aim of this study is to determine the underlying apoptosis molecular and cellular mechanisms of ATO in the presence or absence of ionizing radiation (IR) in vitro in the glioblastoma multiforme (GBM) cell line, U87MG. Methods: Cells were treated by different concentrations of ATO either in presence or absence of IR. Viability and apoptosis pathway of both treated and control groups were evaluated using MTT assay and the expression analysis of Bax, Bcl-2, and caspase-3 genes, respectively. All treatments were performed on 100-μm diameter spheroids. Results: Results showed a significant reduction in the survival of the cells in all treated groups. As expected, cell survival was much less in combination treatment than treatment with only ATO. Moreover, combination therapy made Bax and caspase-3 up-regulated and Bcl-2 down-regulated. Conclusion: ATO and radiation had a synergistic apoptotic effect on GBM cells by up-regulation of caspase-3 and alteration of the Bax-Bcl-2 balance; therefore, ATO may act as a potential anti-cancer agent against GBM cells through triggering the mitochondrial pathway of apoptosis.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Synergistic Effects of Arsenic Trioxide and Radiation: Triggering the Intrinsic Pathway of Apoptosis

Background Arsenic trioxide (ATO) has been reported as an effective anti-cancer and a US Food and Drug Administration (FDA) approved drug for treatment of some cancers. The aim of this study was to determine the underlying apoptosis molecular and cellular mechanisms of ATO in the presence or absence of ionizing radiation (IR) in vitro in the glioblastoma multiforme (GBM) cell line, U87MG. Met...

full text

the effects of planning on accuracy and complexity of iranian efl students’ written narrative task performance

this study compared the different effects of form-focused guided planning vs. meaning-focused guided planning on iranian pre-intermediate students’ task performance. the study lasted for three weeks and concentrated on eight english structures. forty five pre-intermediate iranian students were randomly assigned to three groups of guided planning focus-on-form group (gpfg), guided planning focus...

15 صفحه اول

the effects of time planning and task complexity on accuracy of narrative task performance

هدف اصلی این تحقیق بررسی تاثیر برنامه ریزی زمانی، هم چنین افزایش میزان پیچیدگی تکالیف در نظر گرفته شده بصورت همزمان، بر دقت و صحت و پیچیدگی عملکرد نوشتاری زبان آموزان می باشد. بدین منظور، 50 نفر از دانش آموزان دختر در رده ی سنی 16 الی 18 سال به عنوان شرکت کنندگان در این زمینه ی تحقیق در نظر گرفته شدند و به دو گروه آزمایشی و کنترل بصورت اتفاقی تقسیم شدند. اعضای گروه آزمایشی هر دو تکلیف ساده و پی...

the effects of previewing and providing background knowledge on iranian university efl learners’ comprehension of literary and academic texts

abstract poor reading comprehension can result in failure in using references, benefiting from professional gatherings and resources, keeping up with the growing body of knowledge in the virtual world of the internet, and failing to achieve in efl programs. the purpose of the present quasi-experimental study was to explore the effects of background knowledge and previewing narrative and expos...

15 صفحه اول

the effects of speech rate,prosodic features, and blurred speech on iranian efl learners listening comprehension

کلید واژه ها به زبان انگلیسی: effect of speech rate on listening comprehension, blurred speech,segmental and suprasegmental features,authentic speech,intelligibility, discrimination, omission, assimilation چکیده: سرعت مطالب شنیداری در کلام پیوسته بطور کلی همواره کابوسی بوده برای یادگیرنده های زبان دوم و بالاخص برای شنوندگان ایرانی. علی رغم عقل سلیم که کلام با سرعت کندتری فعالیتهای درک مطلب شن...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 21  issue 5

pages  330- 337

publication date 2017-09

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023